Volume 07,Issue 02

Mycoremediation of Trichoderma harzianum and Penicillium chrysogenum to Pb Exposure: Effect on Metal Bioaccumulation, Oxidative Stress and Antioxidant System

Authors

Semra Malkoc, Hulyam Kurt, Cansu Ozbayer, Emine Yagci

Abstract
One of the biggest problems of the global world is environmental pollution. Much work is being done to prevent environmental pollution and expenditures are being made for this area. The use of biological molecules instead of physical and chemical methods for the removal of metals from industrial water is an alternative, economical and highly effective method. Metal tolerance and detoxification mechanisms in fungi, like many other microorganisms, with bioaccumulation ability to heavy metals, were quite a little knowledge. In this study, the relationship between lead (Pb) bioaccumulation and oxidative stress and antioxidant defenses of T. harzianum and P. chrysogenum was investigated. In order to determine the minimum heavy metal tolerance to lead in the first stage, PDA media containing lead at different concentrations were prepared and fungi growth. It has been observed that as the metal content in the medium increases, the number of fungi colonies developed decreases. Total antioxidant / oxidant levels were then measured, respectively, by TAS (Total Antioxidant Status) Assay Kit and TOS (Total Oxidant Status) Assay Kit and OSI (Oxidative Stress Index) values were calculated. Low OSI values (<0.4) suggest that T. harzianum and P. chrysogenum inhibits lead by tolerating lead.

Keyword: Bioaccumulation, Lead, Penicillium chrysogenum, Trichoderma harzianum, Total oxidant.

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